Products - 3D Cell
Expansion System
A Novel Integrated 3D Perfusion Bioreactor/Incubator System From 3D Biotek!
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White Paper
The 3D Cell Expansion System (3D-CES) is an integrated system designed for large
scale expansion of anchorage-dependent cells in a 3-dimensional (3D)
microenvironment. Culture and expansion of a large quantity of cells by the
traditional two dimensional (2D) method is difficult and known to have many
challenges. The 3D-CES is designed to lift this inherent limitation of 2D cell
culture and expansion methods. With the 3D-CES, cells are grown and expanded on
3D polystyrene (PS) scaffolds. The unique dynamic system circulates media
throughout the system using a peristaltic pump providing an efficient exchange
of nutrients and waste between media and the cells. With minimum hands-on time,
this innovative technology is more efficient and significantly reduces the
amount of time, space, and labor needed to achieve the desired number of cells
in a shorter amount of time compared to other methods.
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Highlight of the major Features
- Integrated Bioreactor/Incubator System for large-scale cell expansion
- Optimized for Stem Cell (Adipose-Derived & Bone Marrow) expansion
- Cells are seeded and grown on 3D Biotek's polystyrene scaffolds
- No need to change media in the entire expansion process
- Reduced human interaction to minimize chances of contamination
- Each culture chamber is capable of expanding up to 250 million cells in 14-18
days
- Two culture chamber design allows max expansion up to 500 million per production
run
- Great potential for use in stem cell therapy and bio-banking
- Also suitable for use in exosomes/protein production for cosmetic industry
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Culture Chamber and Media Container
- The Culture Chamber and Media Container are placed inside a custom-made incubator which provides tubing connection through its side wall to the pump
- Culture Chamber can hold up to 15 pieces of polystyrene (PS) scaffolds
- Cells are seeded and expanded in the PS scaffold which provides a 3D micro-environment
- The capacity of cells for each culture chamber is about 200 - 300 million
- The Media Container can hold up to 3.5 liter
- For 200 - 300 million of cells, about 2.5 liter of media is needed
- Flow of media is from the Media Container in upward direction to the Culture Chamber through the PS scaffolds
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Polystyrene (PS) Scaffold
- Fabricated by 3D Biotek's micro-fabrication technology
- Its unique feature is 100% connectivity within its structure which allows effective exchange of
nutrients and waste between the media and the cells
- PS scaffold is plasma-treated to enhence cell attachment
- The fiber and pore size are approximately 150 and 200 microns, respectively.
- The dimensions of each scaffold are approximately 105(W)x 66.5(D) x 0.6(H) mm
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2-chamber design It allows a max capacity of about 500 million of cells per single run
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Comptational Fluid Dynamics (CFD) analysis
- CFD is used as a design tool for flow optimization
- It provide important parameters such as flow rate and max shear stress
- Max shear stress is below critical value
- Media flow is fairly uniform through the culture chamber and the scaffolds
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| 3D Cell Expansion System is registered with US FDA as Class I medical device
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For more product/pricing information, please call or email us at
info@3dbiotek.com