Products - 3D Insert with Nanomesh

 3D Insert-PS/PCL (Polystyrene/Polycaprolactone) with Nanomesh is developed by combining the benefit of nanofibers and micro-scale 3D scaffold structure to provide a unique feature for use in tumor and stem cell research applications.

 

Use as simulated basement membrane in single or co-culture 3D in vitro models to better maintain cancer phenotype.

 The 3D Insert with Nanomesh offers both nano- and micro-scale geometry to recreate cancer epithelium and stroma tissue-like conditions. Applications of the 3D Insert Nanomesh are suited for monoculture and co-culture in vitro models for drug screening and epithelial-stromal invasion studies.

 

 100% cell seeding efficiency

Cell retention in applications requiring wet coating of ECM proteins such as laminin, fibronectin, growth factors, etc . Currently, the seeding efficiency of the scaffold is 88-95% under dry conditions. However, seeding efficiency drops when applying wet-protein coating prior to cell seeding.

 

Tissue engineering of bone-related models

Electrospun fibers have shown to cause shape-induced osteogenic differentiation of mesenchymal stem cells. The 3D Insert with nano-mesh design combines 3D environment with nano technology. This product is ideal for use in bone tissue engineering studies.

 

Scientific Facts

  • Electrospun Nanofibers promote osteogenic differentiation of mesenchymal stem cells (MSCs) in the absence of osteogenic factors.
  • Electrospun Nanofibers mimic the geometry and stiffness of natural basement membrane. The basement membrane’s main function is to maintain epithelial polarity in tissues such as breast, prostate, and skin etc. Recent reports showed that polymer-based basement membrane promotes upregulation of certain epithelial-to-mesenchymal transitions (metastasis) markers in breast cancer cells.
  • 3D scaffolds with micron size fibers (150-500 microns) promote the proliferation of regular cells and MSCs.
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