Products - 3D
Insert with Nanomesh
3D Insert-PS/PCL (Polystyrene/Polycaprolactone) with Nanomesh is developed by
combining the benefit of nanofibers and micro-scale 3D scaffold structure to
provide a unique feature for use in tumor and stem cell research applications.
Use as simulated basement membrane in single or co-culture 3D in vitro models to
better maintain cancer phenotype.
The 3D Insert with Nanomesh offers both nano- and
micro-scale geometry to recreate cancer epithelium and stroma tissue-like
conditions. Applications of the 3D Insert Nanomesh are suited for monoculture
and co-culture in vitro models for drug screening and epithelial-stromal
cell seeding efficiency
Cell retention in applications requiring wet coating of ECM proteins such as
laminin, fibronectin, growth factors, etc . Currently, the seeding efficiency of
the scaffold is 88-95% under dry conditions. However, seeding efficiency drops
when applying wet-protein coating prior to cell seeding.
Tissue engineering of bone-related models
Electrospun fibers have shown to cause shape-induced
osteogenic differentiation of mesenchymal stem cells. The 3D Insert with
nano-mesh design combines 3D environment with nano technology. This product is
ideal for use in bone tissue engineering studies.
- Electrospun Nanofibers promote osteogenic differentiation of mesenchymal stem cells (MSCs)
in the absence of osteogenic factors.
- Electrospun Nanofibers mimic the geometry and stiffness of natural basement membrane.
The basement membrane’s main function is to maintain epithelial polarity in tissues such as breast,
prostate, and skin etc. Recent reports showed that polymer-based basement membrane promotes upregulation
of certain epithelial-to-mesenchymal transitions (metastasis) markers in breast cancer cells.
- 3D scaffolds with micron size fibers (150-500 microns) promote the proliferation of regular cells and MSCs.